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KMID : 0191120070220061022
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Journal of Korean Medical Science
2007 Volume.22 No. 6 p.1022 ~ p.1025
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Plasmodium falciparum Cultivation Using the Petri Dish: Revisiting the Effect of the ‘Age’ of Erythrocytes and the Interval of Medium Change
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Kim Young-A
Cha Je-Eun
Ahn Sun-Young
Ryu Seung-Ho
Yeom Joon-Sup
Lee Hyo-Il
Kim Chang-Gyun
Seoh Ju-Young
Park Jae-Won
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Abstract
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Differences in the characteristics of the culture conditions can influence the multiplication rate of Plasmodium falciparum. The Petri dish method is one of the most popular methods of cultivating this parasite. In many previous studies, ideal culture conditions of the Petri dish method were achieved by using erythrocytes collected from blood that had been stored for at least 2 weeks, with daily changes of the medium. In the present study, we studied the multiplication rate of P. falciparum in cultures containing erythrocytes of various ages together with changing the medium at various intervals of time. Our results strongly suggest that the rate of in vitro multiplication of P. falciparum was higher in freshly collected erythrocytes than in aged erythrocytes regardless of the anticoagulant and that when the parasitemia is lower than 8% with a hematocrit of 5%, the medium change interval can be as long as 48 hr without a great reduction in the rate of multiplication.
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KEYWORD
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Plasmodium falciparum, In Vitro Cultivation, The Petri Dish Method, Parasite Multiplication, Age of Erythrocytes, Medium Change Interval
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